A reductive ligation based fluorescent probe for S-nitrosothiols
نویسندگان
چکیده
منابع مشابه
A reductive ligation based fluorescent probe for S-nitrosothiols.
A reductive ligation based fluorescent probe () for the detection of S-nitrosothiols (SNO) was developed. The probe showed good selectivity and sensitivity for SNO.
متن کاملReductive ligation mediated one-step disulfide formation of S-nitrosothiols.
A one-step reductive ligation mediated disulfide formation of S-nitrosothiols was developed. This reaction involves the reaction of the S-nitroso group with phosphine-thioesters to form sulfenamide and thiolate intermediates, which then undergo a fast intermolecular disulfide formation to form stable conjugates. This reaction can be used to design new biosensors of S-nitrosated proteins.
متن کاملComparison of Reductive Ligation‐Based Detection Strategies for Nitroxyl (HNO) and S‐Nitrosothiols
Phosphine-based detection strategies for both nitroxyl (HNO) and S-nitrosothiols (RSNO) were investigated and compared. Phosphorus NMR studies show that azaylides derived from HNO or organic RSNO efficiently participate in subsequent reductive ligation required for fluorescence generation in properly substituted substrates. S-Azaylides derived from biological RSNO containing free amine and carb...
متن کاملCover Picture: Comparison of Reductive Ligation‐Based Detection Strategies for Nitroxyl (HNO) and S‐Nitrosothiols (ChemistryOpen 2/2016)
The front cover picture shows a prefluorescent phosphine-based probe reacting with nitroxyl (HNO) and S-nitrosothiol (RSNO), nitrogen oxide-derived biological signals. Both species react with the prefluorescent probe, but only the product from the HNO reaction can complete a further chemical ligation pathway that results in fluorescence, indicating the presence of HNO. The product of the probe ...
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Background: The objective of present study was to assess potential of DHR123 (dihydrorhodamine 123) fluorescent probe for measurement of H2O2 produced by human spermatozoa and comparing the results with DCFH-DA (2', 7'-dichlorodihydrofluorescein diacetate). Materials and Methods: Fluorescent intensity and percentage R123 and DCF positive sperm measured by flow cytometry. The suitable ...
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ژورنال
عنوان ژورنال: Chem. Commun.
سال: 2014
ISSN: 1359-7345,1364-548X
DOI: 10.1039/c4cc01288g